Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Oct 1;217(10):3368–3381. doi: 10.1083/jcb.201802144

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Sirka et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 1. — Luminal-specific Twist1 expression rarely results in dissemination past normal myoepithelium. (A) Mosaic or luminal-specific expression of Twist1 was achieved in K8::Cre-ER;R26::LSL-rtTA;TRE-Twist1 organoids by Adeno-Cre and a tamoxifen (Tam)-inducible Cre-ER, respectively, followed by culture in doxycycline (Dox) to induce Twist1 expression. (B) K8::Cre-ER;R26::LSL-rtTA;TRE-Twist1 organoids from the same mouse were left untreated or treated with Adeno-Cre (Mosaic-Twist1) or tamoxifen (Luminal-Twist1). The number of disseminated cells per organoid was significantly higher in Adeno-Cre–treated organoids compared with tamoxifen-treated organoids (fivefold difference), whereas untreated organoids showed no dissemination across three biological replicates. (C–C′′) The no-Twist1 control showed branching and no dissemination. (D–D′′) Mosaic-Twist1 expression induced branching and dissemination. (E–E′′) Luminal-Twist1 expression rarely led to dissemination. In the Mosaic-Twist1 model, dissemination of both luminal and myoepithelial cells was observed (D′ and D′), whereas in the luminal-Twist1 model, we only observed dissemination of luminal cells (E′ and E′′). n, total number of organoids; r, number of biological replicates. In D and E, red arrowheads mark all disseminated cells. In D′, D″, E′, and E″, red arrowheads mark K14⁻ cells, and green arrowheads mark K14⁺ cells. Data were analyzed by two-tailed nonparametric Kruskal–Wallis multiple comparisons test; ****, P < 0.0001. Data are presented as box plots, with error bars representing the 5th to 95th percentile.