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. 2018 Oct 1;217(10):3368–3381. doi: 10.1083/jcb.201802144

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© 2018 Sirka et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure 2. — The myoepithelium dynamically restrains protruding Twist1⁺ luminal cells. (A) Organoids isolated from K8::Cre-ER;R26::LSL-rtTA;TRE-Twist1;mT/mG mice were treated with tamoxifen (Tam) to induce rtTA expression and cultured in doxycycline to induce Twist1 expression specifically in the luminal cell population. (B) Luminal cell restraint by myoepithelial cells quantified across multiple replicates was observed 92% of the time, and luminal cell escape was observed 8% of the time. (C and C′) Luminal cells were very rarely observed to disseminate past the myoepithelium. (D and D′) Luminal cells that protruded past the myoepithelium were typically restrained by myoepithelial cells. Adjacent myoepithelial cells migrated over and appeared to contain protruding luminal cells. n, total number of videos analyzed; r, number of biological replicates. Myoepithelial cells in time-lapse videos are pseudocolored in green throughout the figure.