Figure 9.

For2A activity is elevated in Δmyo8 cells. For2A-GFP particles were imaged in WT and Δmyo8 cells with VAEM. Particles were tracked with TrackMate. (A) A snap shot from the tracking results. Colored lines are trajectories identified by TrackMate. Bar, 2 µm. (B) Kymographs generated from colored lines in A. Bars, 2 µm (horizontal) and 2 s (vertical). (C) Particle speeds calculated from tracking results were compared with particle speeds measured from kymograph analysis. (D and E) Fraction of immobile For2A-GFP trajectories (D) and the number of linear trajectories per µm² per minute (E) is plotted for WT cells, WT cells treated with 25 µM formin inhibitor SMIFH2, and Δmyo8 cells. Letters a, b, and c indicate statistical groups with α < 0.05 from an ANOVA analysis. (F) Histograms of trajectory length comparing WT and Δmyo8 cells. Data are cumulative from 20 WT cells and 12 Δmyo8 cells. Total trajectories: 960 (WT) and 876 (Δmyo8). Inset, average trajectory length from each cell. The asterisk (*) indicates statistical significance with α < 0.05 from an ANOVA analysis.