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. 2018 Oct 1;217(10):3497–3511. doi: 10.1083/jcb.201709174

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© 2018 Lu et al.

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Figure 4. — Genetic redundancy between streaming and transport ensures the patterning for future embryonic development. (A and A’) Pole cell specification at the posterior side of a syncytial blastoderm embryo from a WT mother shown as a cluster of cells segregated from the somatic cells (A, arrowhead) and positive for anti-Vasa staining (A’, arrowhead). (B and B’) No pole cell specification in a syncytial blastoderm embryo from a Khc-RNAi mother. Bars, 50 µm. DIC, differential interference contrast. (C) Summary of pole cell specification percentages from the listed maternal genotypes. Asterisk marks a percentage that may not be an accurate estimate of pole cell specification percentage as some embryos showed apoptotic/necrotic blebbing and did not reach the syncytial blastoderm stage.