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. 2018 Oct 1;217(10):3593–3607. doi: 10.1083/jcb.201804111

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© 2018 Bean et al.

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Figure 4. — A central region of Vps13 binds Ypt35 in a PxP motif–dependent manner. (A) Schematic of Vps13 truncations. (B) Coimmunoprecipitation of Ypt35-3HA with full-length and truncated forms of Vps13-GFP. Both proteins were overexpressed (OE) from the ADH1 promoter. (C) Densitometry of B; n = 3. (D) Coimmunoprecipitation of WT or mutant Ypt35-3HA with Vps13-GFP fragments indicates that interactions with DUF1162-containing regions are proline dependent. IP, immunoprecipitation; WCL, whole-cell lysate. (E) Densitometry of D; n ≥ 2. (F) Schematic of S. cerevisiae Vps13, highlighting an extended DUF1162 domain defined by six repeats identified by using HHrepID (Biegert and Söding, 2008). (G) An alignment of the most conserved section of the S. cerevisiae DUF1162 repeats. Error bars indicate SEM.