Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Bacillus subtilis) |
PY79 | Youngman et al. (1984) | Wild type | |
| Strain, strain background (Bacillus subtilis) |
GG7 | This paper | amyE::Phyperspank-gpsBSa spec | |
| Strain, strain background (Bacillus subtilis) |
GG8 | This paper | amyE::Phyperspank-gpsBSa-gfp spec | |
| Strain, strain background (Bacillus subtilis) |
GG35 | This paper, derived from FG345, Gueiros-Filho and Losick (2002) |
ΔezrA::spec::erm amyE::Phyperspank-gpsBSa spec | |
| Strain, strain background (Bacillus subtilis) |
CS26 | This paper, derived from BKE22320 (BGSC) |
ΔponA::erm amyE::Phyperspank-gpsBSa spec | |
| Strain, strain background (Bacillus subtilis) |
CS24 | This paper, derived from BKE15770 (BGSC) |
ΔprkC::erm amyE::Phyperspank-gpsBSa spec | |
| Strain, strain background (Bacillus subtilis) |
CS40 | This paper | ΔgpsB::tet amyE::Phyperspank-gpsBSa spec | |
| Strain, strain background (Bacillus subtilis) |
CS94 | This paper, derived from KR546, Ramamurthi and Losick (2009) |
ΔdivIVA::erm amyE::Phyperspank-gpsBSa spec | |
| Strain, strain background (Bacillus subtilis) |
GG9 | This paper, derived from AD3007, Eswaramoorthy et al. (2011) |
amyE::Phyperspank-gpsBSaspec ftsAZΩftsAZ-gfp erm | |
| Strain, strain background (Bacillus subtilis) |
GG18 | This paper | amyE::Phyperspank-gpsBBs spec | |
| Strain, strain background (Bacillus subtilis) |
GG19 | This paper | amyE::Phyperspank-gpsBBs-gfp spec | |
| Strain, strain background (Bacillus subtilis) |
PE448 | This paper | amyE::Phyperspank-gpsBSa-L35S-gfp spec | |
| Strain, strain background (Staphylococcus aureus) |
SH1000 (aka PL3055) | Horsburgh et al. (2002) | Wild type | |
| Strain, strain background (Staphylococcus aureus) |
SH1000 pCL15 | Luong and Lee (2006) | bla cat | |
| Strain, strain background (Staphylococcus aureus) |
SH1000 pPE45 | This paper | pCL15 backbone, Pspac-gpsBSa bla cat | |
| Strain, strain background (Staphylococcus aureus) |
SH1000 pPE83 | This paper | pCL15 backbone, Pspac-gpsBBs bla cat | |
| Strain, strain background (Staphylococcus aureus) |
SH1000 pPE79 | This paper | pCL15 backbone, Pspac-gpsBSa-L35S bla cat | |
| Strain, strain background (Staphylococcus aureus) |
SH1000 pPE46 | This paper | pCL15 backbone, Pspac-gpsBSa-gfp bla cat | |
| Strain, strain background (Staphylococcus aureus) |
SH1000 pPE80 | This paper | pCL15 backbone, Pspac-gpsBSa-L35S-gfp bla cat | |
| Strain, strain background (Staphylococcus aureus) |
SH1000 pEPSA5 | Forsyth et al. (2002) | bla cat | |
| Strain, strain background (Staphylococcus aureus) |
SH1000 pGG59 | This paper | pEPSA5 backbone,Pxyl-gpsBantisense bla cat | |
| Strain, strain background (Staphylococcus aureus) |
SH1000 pRB42 | This paper | pJB67 backbone, PCd-zapASa-gfp bla erm | |
| Sequence-based reagent (oligonucleotide) |
oP36 | This paper | AAAAAGCTTACATAA
GGAGGAACTACTATGTCAGATGTTTCATTGAAATTATCAGCA |
|
| Sequence-based reagent (oligonucleotide) |
oP37 | This paper | AAAGCTAGCTTTACCA AATACAGCTTTTTCTAAGTTTGA |
|
| Sequence-based reagent (oligonucleotide) |
oP38 | This paper | AAAGCATGCTTATTTACCAAATACAGCTTTTTCTAAGTTTGA | |
| Sequence-based reagent (oligonucleotide) |
oP46 | This paper | AAAGCTAGCATGAGTAAAGGAGAAGAACTTTTC | |
| Sequence-based reagent (oligonucleotide) |
oP24 | This paper | GCCGCATGCTTATTTGTATAGTTCATCCATGCC | |
| Sequence-based reagent (oligonucleotide) |
oP100 | This paper | AAAGTCGACACATA
AGGAGGAACTACTATGCTTGCTGAT AAAGTAAAGCTTTCTGCG |
|
| Sequence-based reagent (oligonucleotide) |
oP101 | This paper | AAAGCTAGCATCA TAAAGCTTGCTGCCAAAAACGTG |
|
| Sequence-based reagent (oligonucleotide) |
oP102 | This paper | AAAGCTAGCTCAAT CATAAAGCTTGCTGCCAAAAACGTG |
|
| Sequence-based reagent (oligonucleotide) |
oP195 | This paper | AAAGGATCCTCAATCATAAAGCTTGCTGCCAAAAACGTG | |
| Sequence-based reagent (oligonucleotide) |
oP187 | This paper | AAAGAATTCTTATTTACCAAATACAGCTTTTTCTAAGTTTGAAATACGTTTTAAAATATCTAC | |
| Sequence-based reagent (oligonucleotide) |
oP188 | This paper | AAAGGATCCGAGG TGGAAAAAATGTCAGATGTTTCATTGAA ATTATCAGC |
|
| Sequence-based reagent (oligonucleotide) |
oP236 | This paper | AAAGTCGACTAATGAGGAGGAAAAAATGACACAGTTTAAAAACAAGGTAAATGTATCAATTAATGATCAG | |
| Sequence-based reagent (oligonucleotide) |
oP237 | This paper | AAAGCTAGCCGCTGCTG CAATTTGTGAATTTGTTGTTTCAAACGT |
|
| Sequence-based reagent (oligonucleotide) |
oP47 | This paper | AAAGGATCCTTATTTGTATAGTTCATCCATGCC | |
| Antibody | anti-GpsB | This paper | Raised against purified GpsB-His6 | |
| Antibody | anti-SigA | Ramamurthi lab | Raised against purified B. subtilis SigA-His6 |