Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Y. lipolytica GB20) |
∆mus51, lys1-, leu2-, ura3-, 30Htg2, ndh2i |
PMID 24706851 | ||
| Strain, strain background (E. coli CLY) |
derived from C43(DE3), ∆cyoBCD::kan |
PMID 17267395 | Prof. Robert B. Gennis, University of Illinois |
|
| Genetic reagent (pET17b-V14) |
cyoABCDE in pET 17b |
this work | Dr. Hao Xie, MPI for Biophysics, Frankfurt |
|
| Chemical compound, drug |
n-Dodecyl β- maltoside |
Glycon Biochemicals GmbH |
Cat. # D97002-C | |
| Chemical compound, drug |
Decylubiquinone | Sigma-Aldrich/Merck | Cat. # D7911 | |
| Chemical compound, drug |
β-Nicotinamide adenine dinucleotide |
Sigma-Aldrich/Merck | Cat. # N8129 | |
| Chemical compound, drug |
Asolectin from soybean |
Sigma-Aldrich/Merck | Cat. # 11145 | |
| Chemical compound, drug |
CHAPS, Anagrade | Anatrace | Cat. # C316 | |
| Software, algorithm |
Coot | PMID: 15572765 | RRID: SCR_014222 | |
| Software, algorithm |
CTFFIND4 | PMID:26278980 | ||
| Software, algorithm |
Gctf | PMID:26592709 | ||
| Software, algorithm |
MolProbity | PMID: 20057044 | RRID: SCR_14226 | |
| Software, algorithm |
MotionCor2 | PMID: 28250466 | ||
| Software, algorithm |
Phenix | PMID: 20124702 | RRID: SCR_014224 | |
| Software, algorithm |
PyMOL | Schrödinger, LLC | RRID: SCR_000305 | |
| Software, algorithm |
RELION | PMID: 27845625 | RRID: SCR_016274 | |
| Software, algorithm |
UCSF Chimera | PMID: 15264254 | RRID: SCR_004097 | |
| Software, algorithm |
TMHMM Server | PMID: 11152613 | RRID: SCR_014935 |