Table 1. Data collection, refinement and model statistics.
| Deactive | steady-state turnover | |
|---|---|---|
| Data collection Microscope |
FEI Tecnai Polara |
FEI Titan Krios |
| Camera | Gatan K2 Summit | Falcon III |
| Voltage (kV) Nominal magnification Calibrated pixel size (Å) |
300 200,000x 1.09 |
300 75,000x 1.053 |
| Total exposure (e-/Å2) | 60.5 | 30.7–40.7 |
| Exposure rate (e-/pixel/s) Number of frames |
9 40 |
0.4 81 |
| Defocus range (μm) | 1.5–3.0 | 1.5–4.5 |
| Image processing Motion correction software |
MotionCor2 |
MotionCor2 |
| CTF estimation software | CTFFIND4 | Gctf |
| Particle selection software | EMAN boxer and RELION2.1 | RELION2.1 |
| Initial/final micrographs | 3,110/3,110 | 5,964/5,650 |
| Particles selected | 271,443 | 273,581 |
| Applied B-factor (Å2) | −142 | −215 |
| Final resolution (Å) | 4.3 | 4.5 |
| Refinement statistics Modeling software |
COOT, PHENIX |
|
| Number of residues | 7515 | |
| Map CC (whole unit cell) | 0.786 | |
| RMS deviations Bond-lengths (Å) |
0.0099 | |
| Bond-angles (°) | 1.52 | |
| Av. B-factor (Å2) | 151.46 | |
| Ramachandran plot Outliers (%) |
0.74 |
|
| Allowed (%) | 13.43 | |
| Favoured (%) | 85.83 | |
| Rotamer outliers (%) | 0.71 | |
| Molprobity score | 2.11 | |
| All-atom clashscore | 8.55 | |
| PDB ID | 6GCS |