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. 2018 Aug 14;59(10):1880–1892. doi: 10.1194/jlr.M085431

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Copyright © 2018 Madsen et al. Published by The American Society for Biochemistry and Molecular Biology, Inc.

Author’s Choice—Final version open access under the terms of the Creative Commons CC-BY license.

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Fig. 5. — Precursor ion scanning of labeled BALF PC. A: Precursor scan of m/z 193 detailing the incorporation of methyl-D₉-choline into PC species 9 amu greater than unlabeled PC. B: Precursor ion scan of m/z 189 showing the incorporation into PC species of [5¹³C]choline derived from [U¹³C]DPPC. The incorporation of [5¹³C]choline by the CDP-choline pathway is given by odd-numbered species 4 amu lower than in A (e.g., m/z 739.5 vs. 743.6). The addition of an unlabeled acyl chain to [24¹³C]lysoPC16:0 is indicated by even-numbered species (e.g., m/z 806.6 and 830.6). These spectra are from a mouse 48 h after being administered CHF5633 and methyl-D₉-choline.