TABLE 1.
Diagnostic MS/MS scans for the analysis of PC metabolism
| Diagnostic MS/MS Scans for Choline Head Groups | |||
| MS/MS Mode | Lipids | Range (m/z) | Characterization |
| Precursor m/z 184 | PC | 400–900 | PC species containing phosphorylcholine |
| Precursor m/z 189 | [513C]PC | 400–900 | PC species containing [513C]choline |
| Precursor m/z 193 | [D9]PC | 400–900 | PC species containing [D9]choline |
| Diagnostic MS/MS Scans for DPPC with Different Labeled Diacylglycerol Components | ||||
| MS/MS Mode | Lipids | Range (m/z) | Ion (m/z) | Characterization |
| Neutral loss 551 | DPPC | 732–745 | 734.5 | Unlabeled DPPC |
| Neutral loss 554 | [313C]DPPC | 735–748 | 737.5 | DPPC—[313C]glycerol or DPPC M+3+ |
| Neutral loss 567 | [1613C]DPPC | 748–761 | 750.8 | DPPC—one 1613C palmitate |
| Neutral loss 570 | [1913C]DPPC | 751–764 | 753.5 | DPPC—one 1613C palmitate + [313C]glycerol |
| [2413C]DPPC | 758.5 | DPPC—[2413C]lysoPC16:0 + [1612C]palmitate | ||
| Neutral loss 583 | [3213C]DPPC | 764–777 | 766.5 | DPPC—two 1613C palmitates |
| [3713C]DPPC | 771.5 | [U13C]DPPC M-3+ | ||
| Neutral loss 586 | [U13C]DPPC | 767–780 | 774.5 | [U13C]DPPC |
| Diagnostic MS/MS Precursor Ion Scans in Negative Ionization | |||
| MS/MS Mode | Lipids | Range | Characterization |
| Precursor 255 | PC | 400–900 | PC species containing unlabeled palmitate |
| Precursor 271 | [1613C]PC | 400–900 | PC species containing [1613C]palmitate |