Roles of Ang II on the promoter activity of ABCA1 in INS-1 cells. A: The effects of a PI3K inhibitor (LY), a MEK inhibitor (PD), a p38 MAPK inhibitor (SB), a PKA inhibitor (H-89), or CaMKK inhibitor STO609 (STO) with or without Ang II on ABCA1 promoter activity. Percentage of promoter activity is referred to control. B: Phosphorylation of MEK at Ser217/221 and ERK at Thr202/Tyr204 in INS-1 cells treated with Ang II. C: The expression of ABCA1 in INS-1 cells treated with PD and Ang II. The ratio of ABCA1 to GAPDH is shown as percent of control. A graph showing the mean ± SEM (n = 3) of separate experiments for each treatment group is included. Control, no Ang II; Ang II, Ang II at 10 nM; Ang II+PD, Ang II at a concentration of 10 nM plus PD at a concentration of 10 µM. * P < 0.05 compared with control (0); #
P < 0.05 compared with DMSO plus Ang II.