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. 2018 Aug 14;59(10):1906–1915. doi: 10.1194/jlr.M085886

Fig. 5.

Fig. 5.

Ang II regulates the expression of ABCA1 via its receptor, AT1R in INS-1 cells. A, B: The expression of Ang II receptor AT1 (A) and AT2 (B). C: ABCA1 expression in INS-1 cells treated with or without AngII and the inhibitor of AT1R, RNH-6270 at varying concentration (0, 10, or 100 nM). The ratio of ABCA1 to GAPDH is shown as percent of control. D: The effect of RNH on Ang II-suppressed ABCA1 promoter activity. Percentage of promoter activity is referred. E: Phosphorylation of ERK and MEK in INS-1 cells treated with RNH-6270 (10 nM) and Ang II (10 nM). F, G: Cholesterol content and Oil Red O stain in INS-1 cells treated with RNH-6270 and Ang II. F: Percentage of cholesterol content per cell is referred to control. A graph showing the mean ± SEM (n = 3) of separate experiments for each treatment group is provided. G: Oil Red O stain. The images in the upper row were 200× magnification (bar = 20 µm), and those in the lower row were 400× magnification (bar = 10 µm). Ang II, Ang II at a concentration of 10 nM; RNH, RNH-6270 at a concentration of 10 nM. * P < 0.05 compared with control; # P < 0.05 compared with Ang II.