TABLE 1.
Bacterial strains and plasmids used in this study
| Strains and plasmids | Relevant characteristics | Source/reference |
| Strains | ||
| Bacillus subtilis | ||
| JH642 | trpC2 pheA1 | Laboratory stock |
| LC5 | JH642 des::Kmr | (8) |
| EV | LC5 amyE::pSG1154 Kmr Spr | (19) |
| DesA | LC5 amyE::Pxil-desA Kmr Spr | (19) |
| Des | LC5 amyE::Pxil-des Kmr Spr | This study |
| BL02692 | LC5 amyE::Pxil-ORFBL02692 Kmr Spr | This study |
| Chimera I | LC5 amyE::Pxil-(desA-des) Kmr Spr | This study |
| Chimera II | LC5 amyE::Pxil-(des-desA) Kmr Spr | This study |
| Chimera III | LC5 amyE::Pxil-(desA-ORFBL02692) Kmr Spr | This study |
| Chimera IV | LC5 amyE::Pxil-(ORFBL02692-desA) Kmr Spr | This study |
| Chimera V | LC5 amyE::Pxil-(desAN1-ORFBL02692(N2+C)) Kmr Spr | This study |
| BL02692 Cys40Tyr | LC5 amyE::Pxil- ORFBL02692 (C40Y) Kmr Spr | This study |
| BL02692 Cys40Ala | LC5 amyE::Pxil- ORFBL02692 (C40A) Kmr Spr | This study |
| DesA Tyr40Cys | LC5 amyE::Pxil-desA (Y40C) Kmr Spr | This study |
| DesA Gly90Arg | LC5 amyE::Pxil-desA (G90R) Kmr Spr | This study |
| DesA Trp104Arg | LC5 amyE::Pxil-desA (W104R) Kmr Spr | This study |
| DesA Arg149Ala | LC5 amyE::Pxil-desA (R149A) Kmr Spr | This study |
| DesA Lys172Ala | LC5 amyE::Pxil-desA (K172A) Kmr Spr | This study |
| DesA Gly201Ala | LC5 amyE::Pxil-desA (G201A) Kmr Spr | This study |
| DesA His228Ala | LC5 amyE::Pxil-desA (H228A) Kmr Spr | This study |
| DesA Pro257Ala | LC5 amyE::Pxil-desA (P257A) Kmr Spr | This study |
| DesA Leu275Arg | LC5 amyE::Pxil-desA (L275R) Kmr Spr | This study |
| DesA Pro277Ala | LC5 amyE::Pxil-desA (P277A) Kmr Spr | This study |
| DesA Tyr282Arg | LC5 amyE::Pxil-desA (Y282R) Kmr Spr | This study |
| DesA Leu284Arg | LC5 amyE::Pxil-desA (L284R) Kmr Spr | This study |
| DesA Tyr26Trp, Ile28Leu | LC5 amyE::Pxil-desA (Y26W-I28L) Kmr Spr | This study |
| DesA Phe51Trp | LC5 amyE::Pxil-desA (F51W) Kmr Spr | This study |
| Escherichia coli DH5α | supE44 thi-1 ΔlacU169 ϕ80lacZ ΔM15 endA1 recA1 hsdR17 gyrA96 relA1 trp6 cysT329::lac inmλpI(209) | Laboratory stock |
| Plasmids | ||
| pCR-Blunt II-Topo | E. coli cloning vector, Kmr | Invitrogen |
| pGEM-T Easy | E. coli cloning vector, Ampr | Promega |
| pSG1154 | Expression vector that integrates at the amyE locus of B. subtilis, Spr | (16) |