TABLE 3.

Measurements of sterols analyzed following WT-N2a cells’ exposure to DHCR24 inhibitors for 24 h at the indicated concentrations

Sterol	Vehicle	Masitinib (nM)			Ponatinib (nM)			Pregnenolone (nM)
		62	125	1,000	62	125	1,000	62	125	1,000
Lan	0.13 ± 0.01	0.11 ± 0.02	0.11 ± 0.01	0.31 ± 0.03	0.15 ± 0.03	0.15 ± 0.03	0.17 ± 0.02	0.18 ± 0.06	0.15 ± 0.01	0.10 ± 0.02
DHLan	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.
Zym	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.
Zyme	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.
24-DHL	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.
Lath	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.
7-DHD	0.21 ± 0.05	0.28 ± 0.06	0.29 ± 0.02	0.37 ± 0.17	0.29 ± 0.14	0.25 ± 0.18	0.50 ± 0.36	0.51 ± 0.23	0.32 ± 0.03	0.42 ± 0.03
7-DHC	0.13 ± 0.01	0.13 ± 0.02	0.12 ± 0.02	0.13 ± 0.01	0.11 ± 0.02	0.07 ± 0.02	0.13 ± 0.05	0.12 ± 0.02	0.13 ± 0.02	0.1 ± 0.01
Des	6.2 ± 0.16	7.7 ± 0.41a	8.0 ± 0.50a	5.9 ± 0.22	8.8 ± 0.79a	7.0 ± 0.71	4.4 ± 0.21a	9.1 ± 0.36a	11 ± 0.54a	8.8 ± 0.29a
Chol	13 ± 0.28	11 ± 0.80a	8.9 ± 0.53a	9.1 ± 0.24a	9.4 ± 0.69a	8.8 ± 0.50a	12 ± 0.51a	8.9 ± 0.42a	9.5 ± 0.25a	8.9 ± 0.73a

Data are expressed as mean ± SEM. Zyme, zymostenol; Lath, lathosterol; n.d., not detected.

^aSignificant difference to control, P < 0.05.