Negative-ion MALDI-TOF spectra and LOS profiles of C. concisus and C. jejuni LOS. A–E: Negative-ion MALDI-TOF MS spectra for the LOS of C. concisus isolates NCTC 12408, B38, 2010-131105, 2010-347972, and C. jejuni 11168H, respectively. The relative abundance of the peaks was magnified where indicated. The m/z values presented are for the monoisotopic peaks observed (red font for lipid A fragments, blue font for OS fragment ions, and black font for molecular ions). Asterisks show peaks corresponding to sodiated molecular or fragment ion peaks. Purified LOS (10 μg per lane) of C. concisus isolates NCTC 12408, B38, 2010-131105, 2010-347972, and C. jejuni 11168H were separated by SDS-PAGE and stained using silver nitrate. F: The gel was cropped where indicated (black lines).