Analysis of intact LOS from C. concisus B38 by MALDI-TOF MS with IMS. The panel shows a 2D plot of the IMS data set. A: Differences in drift time allow for the separation of low-molecular-weight components of non-LOS origin, OS prompt fragment ions, intact LOS, and lipid A prompt fragment ions. B: Regions of the mass spectra of the (M-H)− molecular ions, lipid A prompt fragments, and OS prompt fragments. Spectra were acquired with the Synapt G2 high-definition mass spectrometer using IMS and analyzed with DriftScope and MassLynx software.