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. Author manuscript; available in PMC: 2019 Oct 2.
Published in final edited form as: Anal Chem. 2018 Sep 19;90(19):11572–11580. doi: 10.1021/acs.analchem.8b02913

Figure 3.

Figure 3.

Influence of MALDI MSI on IR imaging data quality for rat hippocampus images. (A) Discrete frequency IR images of the 1248 cm−1 and 1656 cm−1 bands of adjacent tissue sections subjected to either (top) PFA treatment, (middle) sublimation of DHB with subsequent PFA treatment, or (bottom) sublimation of DHB and MALDI MSI analysis, with subsequent PFA treatment. (B) The average absorbance spectra from the color-coded regions of the hippocampus are overlaid. While there are several morphological differences between the three tissue sections, the IR spectra match.