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. Author manuscript; available in PMC: 2018 Oct 3.
Published in final edited form as: Leukemia. 2018 Apr 2;32(11):2363–2373. doi: 10.1038/s41375-018-0124-y

Figure 6. Activation of MYC by FGFR1 kinases leads to upregulation of MYC target genes.

Figure 6

GSEA analysis of RNASeq data from BBC2, ZNF112 and BCRF8C following treatment with the BGJ398 FGFR1 inhibitor shows statistically significant (A) downregulation of MYC target genes. The majority of genes up-regulated upon MYC overexpression show reduced expression following BGJ398 treatment. The expression levels of MYC and three selected targets of MYC (NCL, ODC1 and CDK4), as determined by qPCR, shows down regulation (55-85%) following BGJ398 treatment in all 4 cell lines expressing the chimeric kinases (C, above). Focused analysis of a wider selection of MYC target genes in the human KG1 cell line (for which RNASeq data is not available) also shows consistent down regulation (C, below). 50% reduction in expression levels is shown by the broken lines in each case.