Fig. 1.

Cryo-EM reconstruction and atomic model of the BCL10-MALT1 filament. a Domain organization of the BCL10 and MALT1 protein constructs. b Example cryo-EM micrograph of the BCL10-MALT1 complex filaments. c, d 2D class averages and corresponding power spectra of BCL10-MALT1 filaments used for helical parameter determination. e, f Overall architecture of the BCL10-MALT1 filament assembly shown together with the cryo-EM density, clipped top and side view, respectively. The map shows the inner well-structured part of the filament at an overall resolution of 4.9 Å (FSC curve see Supplementary Fig. 8a). This part consists of the BCL10 CARD (residues 10–115) and the MALT1 DD (residues 30–121) colored blue and orange, respectively. g, h Example parts of the cryo EM density map shown together with the BCL10-MALT1 DD and the atomic model of BCL10 shown in ribbon and stick representation, respectively. i Overlay of the MALT1 DD cryo-EM and crystal structure (pdb ID: 2g7r) colored orange and beige, respectively