Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Oct 2;8:14675. doi: 10.1038/s41598-018-32821-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Arc and PICK1 form complexes in live cells. (a–c) Intensity images of a representative SH-SY5Y cell co-transfected with Arc-mCherry and EGFP-PICK1 show co-localization in puncta. (d–f) ccRICS analysis demonstrates directed interaction of Arc-mCherry and EGFP-PICK1 in live cells. Cross-correlation of the fluctuation from Arc and PICK1 (d,e) produced a new correlation curve (f) of only the fluorophores which are moving together in the cells. The curve is smaller because the correlated signal is only a fraction of the total particles in each channel. Diffusion obtained in the correlated sample is similar to that obtained from individual RICS analysis. Lack of interaction would be predicted to produce a randomized, non-correlated signal which was observed for our control conditions.