Table 1.
Fluctuation analysis of cytosolic Arc-mCherry and EGFP-PICK1.
| Transfected Protein (s)a | Ave. Diffusion (µm2/sec)b | Ave. ε (cpsm) | Norm. εd |
|---|---|---|---|
| EGFP | 9.0 ± 3.0 | 4,500 ± 500 | 1.00 |
| EGFP-PICK1 + mCherry | 1.0 ± 0.9 | 6,200 ± 1,200 | 1.38 |
| EGFP-PICK1 + Arc-mCherry | 1.4 ± 0.7 | 6,300 ± 1,100 | 1.40 |
| mCherry | 9.1 ± 2.5 | 3,500 ± 500 | 1.00 |
| Arc-mCherry + EGFP | 1.9 ± 0.9 | 3,900 ± 1,100 | 1.11 |
| Arc-mCherry + EGFP-PICK1 | 1.1 ± 0.7 | 4,800 ± 1,500c | 1.37c |
aFor each condition, n > 20 with concentrations of transfected proteins ranging from 20 to 1,500 nM. No significant trend was found for concentration and thus each concentration was included in the final calculations.
bp < 0.05 from a multivariate ANOVA using Bonferroni post-hoc when control samples, EGFP and mCherry, where compared to the experimental conditions.
cp < 0.05 from a multivariate ANOVA using Bonferroni post-hoc when control sample, mCherry, was compared to Arc-mCherry + EGFP-PICK1.
dValues were generated by dividing by monomer controls to each corresponding sample.