Table 2.
Average fluorescence lifetimes of EGFP-PICK1 in depolarized and non-depolarized cells.
| Transfected Protein (s)a | Depolarized | Ave. Lifetime 1 (ns) | Ave. Lifetime 2 (ns) | % showing FRET | FRET efficiency |
|---|---|---|---|---|---|
| EGFP | N | 2.41 ± 0.03 | — | — | — |
| EGFP | Y | 2.43 ± 0.01 | — | — | — |
| EGFP-PICK1 | Y | 2.40 ± 0.03 | 1.93 ± 0.05 | 10% | 10% (±6%) |
| EGFP-PICK1 + Arc-mCherry | N | 2.38 ± 0.04 | 1.95 ± 0.01 | 15% | 18% (±9%) |
| EGFP-PICK1 + Arc-mCherry | Y | 2.37 ± 0.05 | 2.00 ± 0.20 | 60% | 28% (±10%) |
aFor each condition, n > 20.
The average lifetime and standard deviation are presented for each group. If two lifetimes were present based on the phasor plot distribution a second average lifetime and standard deviation are presented along with the percentage of cells showing FRET and the FRET efficiency observed.