Table 3.
TIRF N&B analysis of membrane Arc-mCherry and EGFP-PICK1.
| Transfected Proteina | Ave. Brightness (cpsm) | Norm. Brightnessb |
|---|---|---|
| EGFP | 0.10 ± 0.01 | 1.0 |
| EGFP-PICK1 | 0.25 ± 0.05 | 2.5 |
| EGFP-PICK1 + mCherry | 0.22 ± 0.04 | 2.2 |
| EGFP-PICK1 + Arc-mCherry | 0.22 ± 0.05 | 2.2 |
| mCherry | 0.10 ± 0.02 | 1.0 |
| Arc-mCherry | 0.17 ± 0.03 | 1.7 |
| Arc-mCherry + EGFP | 0.17 ± 0.04 | 1.7 |
| Arc-mCherry + EGFP-PICK1 | 0.16 ± 0.03 | 1.6 |
aFor each condition, n > 40.
bValues were generated by dividing by monomer controls to each corresponding sample. We note for Arc-mCherry that there was a trend towards dimer at higher concentration and that this values represents the average self-association for the concentration range tested.