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. 2018 Oct 2;8:14689. doi: 10.1038/s41598-018-32942-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of a conserved Sin3 Interaction Domain (SID) in Tet1 and Tet3. (A) Schematic diagram of Tet1, 2 and 3 showing the presence of a putative helical SID in Tet1/3, but not Tet2. (B) The putative Tet1-SID is highly conserved across multiple species; Hs- Homo sapiens, Mm – Mus musculus, Ne - Notamacropus eugenii (Wallaby), Ps - Pelodiscus sinensis (Chinese soft shell turtle), Dr - Danio rerio. (C) Co-immunoprecipitation followed by western blotting reveals the requirement of the Tet1-SID for association with exogenous and endogenous Sin3A. This image is cropped, the uncropped version of the blot is shown in Supplementary Fig. S5A.