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. 2018 Oct 2;9:4031. doi: 10.1038/s41467-018-06404-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — MARF1^null eggs fail to complete meiosis. a Meiotic spindles show proper meiotic metaphase I arrest in control and MARF1^null stage 14 oocytes. The percentages of normal oocytes with proper meiotic spindle are shown. The numbers of normal oocytes with proper meiotic spindle/the numbers of observed oocytes are shown in parenthesis. Scale bar is 5 μm. b Polar body shows completion of meiosis in control unfertilized eggs, but not in MARF1^null unfertilized eggs. Neither meiotic spindle nor polar body were observed in MARF1^null unfertilized eggs. The percentages of normal unfertilized eggs with a proper polar body are shown. The numbers of normal unfertilized eggs with a proper polar body/the numbers of observed unfertilized eggs are shown in parenthesis. Scale bar is 5 μm. DNA was stained with DAPI (blue) and alpha-Tubulin was visualized with FITC-conjugated anti-alpha-Tubulin antibody (green)