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. 2018 Oct 2;8:14609. doi: 10.1038/s41598-018-32975-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Number and direction of differentially methylated cytosines (DMCs) corresponding to 30 shared differentially methylated genes (DMGs) between liver and mammary gland tissue. Liver was collected from in utero heat stressed (IUHT-B) and in utero cooled (IUCL-B) bull calves at birth. Mammary tissue was collected from in utero heat stressed (IUHT-H) and in utero cooled (IUCL-H) heifers at 21 days into their first lactation. Methylation comparison was made between IUHT and IUCL groups for bull calves and heifers separately. Hypo- or hypermethylation of cytosines refers to IUHT relative to IUCL. Pink bars denote heifer mammary gland, blue bars indicate bull calf liver. Novel genes are genes that have not yet been characterized in the bovine genome. In addition to the 30 shared DMGs shown here, there were an additional 20 shared DMGs that code for rRNAs.