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. 2018 Oct 2;8:14609. doi: 10.1038/s41598-018-32975-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relationship between differential cytosine methylation and differential gene expression in the mammary gland of in utero heat stressed (IUHT-H) and in utero cooled (IUCL-H) heifers. (A) Overall DMCs regardless of their genomic location. There was a non-significant negative correlation between differential methylation and differential gene expression when including DMCs regardless of genomic location (r = −0.12, P = 0.2). (B) Includes only DMCs located in the gene body. There was a tendency for a significant negative correlation between differential methylation and differential gene expression when including only DMCs located in the gene body (r = −0.23, P = 0.09).