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. 2018 Oct 2;8:14605. doi: 10.1038/s41598-018-33038-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of differentially expressed miRNAs. (A) Scatter plots assess the miRNA expression variation between the two compared groups. The miRNAs above the dotted line and below the bottom dotted line indicated more than two-fold changes between the two compared samples. (B) Box plot of log2 (FPKM) values across control and IR miRNAs. Control, normal esophageal tissue sample and IR, ionizing radiation treated sample. (C) Volcano plot of the differentially expressed miRNAs. The vertical lines correspond to 1.5-fold up and down, respectively, and the horizontal line represents P = 0.05. The green and red dots in the plot represent the differentially expressed miRNAs with statistical significance. (D) Heat map showing expression profiles of differentially expressed miRNAs.