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. 2018 Oct 2;8:14679. doi: 10.1038/s41598-018-32762-y

Figure 4.

Figure 4

PKMζ-PKCι/λ is required for amygdala-dependent threat memory maintenance. To test causation, pups were cannulated bilaterally in the amygdala two days before conditioning (a) and administered ZIP or scrambled ZIP 2 hours before Y-maze testing. At baseline (no CORT manipulation), ZIP blocked amygdala-dependent threat memory expression in PN12 and PN16 pups that underwent odor-shock conditioning (b). ZIP administration blocked threat memory expression in pups that learned amygdala dependent threat following CORT manipulation (PN8 + CORT, PN12 + Veh, PN16 + MET, PN16 + Veh, (c). *P < 0.05, **p < 0.01, ***p < 0.001; † denotes significant difference from chance performance on Y-maze (dotted line), p < 0.05. Error bars indicate mean ± SEM. Veh, vehicle; MET, metyrapone; CORT, corticosterone. Representative histology confirming cannula placement is presented in Supplementary Figure 6.