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. 2018 Oct 2;8:14687. doi: 10.1038/s41598-018-32742-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic drawing of the floating bean leaf disc assay. In vitro transcribed dsRNAs were delivered to TSSM through bean leaf disc. 200 μl of dsRNA (300 ng/μl concentration) were placed on the leaf on the first day, followed by adding 50 μl of dsRNA on each day up to nine days.