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. 2018 Oct 2;8:14687. doi: 10.1038/s41598-018-32742-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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dsMet caused knockdown of Met gene expression and mortality of TSSM. (A) dsGFP and dsMet were fed to TSSM for seven days. The TSSM were collected from the bean leaf discs and RNA isolated and used in qRT-PCR to determine the relative Met mRNA levels. The error bars show Mean ± SEM (n = 3). (B) The leaf disc assay was performed to check the effect of feeding dsMet on TSSM. The picture was taken on day 9; the majority of TSSM died during the last molt stage after feeding on Met dsRNA.