Table 2.
Primer name | Sequences (5′~3′) | Amplicon length (bp) |
---|---|---|
GFP_dsRNA_F | CGATGCCACCTACGGCAA | 248 |
GFP_dsRNA_R | TGAAGTTCGAGGGCGACA | |
TuEcR_dsRNA_F | AGCTCCAAGACAGCAAGAAG | 486 |
TuEcR_dsRNA_R | GTCTCTGAGGGAGACTCATGTA | |
TuMet_dsRNA_F | AAGCATCCACCTCGGACATCTCTT | 306 |
TuMet_dsRNA_R | ATTGCGACTCTGGTGTCAGGGAAT | |
TuRXR1_dsRNA_F | TGTCGGGAAGAACGAGATTG | 340 |
TuRXR1_dsRNA_R | CGGGTAACTCGGTGAAATGA | |
TuRXR2_dsRNA_F | GAGGAGCGACAACGGAATAA | 373 |
TuRXR2_dsRNA_R | CGGCTTGATGTGCTGAATTAC | |
TuRXR β _dsRNA_F | TCCGTTTACCGATGCAAGAA | 381 |
TuRXR β _dsRNA_R | GTGGAACGACTCAAGGGTTAT | |
TuSRC_dsRNA_F | CGTGACATGCCGAAGAAGATA | 355 |
TuSRC_dsRNA_R | TACCAAGGGCAGACATAGGA | |
TuCBP_dsRNA_F | ACCCAGTCACCCAATGTATC | 334 |
TuCBP_dsRNA_R | AAGATGGTGGTGGAGTGTATC | |
TuFaMet_dsRNA_F | GGATTGATGCTGAAGGAGGT | 329 |
TuFaMet_dsRNA_R | ATGAGATCCTTGATGGAAGGTG | |
rp49_qRT_F | CTTCAAGCGGCATCAGAGC | 105 |
rp49_qRT_R | CGCATCTGACCCTTGAACTTC | |
TuMet_qRT_F | GGTGCGCTCCGATGAAATCAATGT | 89 |
TuMet_qRT_R | AGCCTAAGCTAGCGAACGCAGAAT | |
TuMet_Trans_F | AGCAAGCTT ATGGCCACTGAGGAAACAATGG | |
TuMet_Trans_R | TCGACTCGAGTTATTGTTTGAGATCTAGTTCGGGT |
T7 promoter sequence TAATACGACTCACTATAGGG was added at the 5′ end of each dsRNA primer.