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. 2018 Oct 2;8:14687. doi: 10.1038/s41598-018-32742-2

Table 2.

Sequences of the primers used in this study.

Primer name Sequences (5′~3′) Amplicon length (bp)
GFP_dsRNA_F CGATGCCACCTACGGCAA 248
GFP_dsRNA_R TGAAGTTCGAGGGCGACA
TuEcR_dsRNA_F AGCTCCAAGACAGCAAGAAG 486
TuEcR_dsRNA_R GTCTCTGAGGGAGACTCATGTA
TuMet_dsRNA_F AAGCATCCACCTCGGACATCTCTT 306
TuMet_dsRNA_R ATTGCGACTCTGGTGTCAGGGAAT
TuRXR1_dsRNA_F TGTCGGGAAGAACGAGATTG 340
TuRXR1_dsRNA_R CGGGTAACTCGGTGAAATGA
TuRXR2_dsRNA_F GAGGAGCGACAACGGAATAA 373
TuRXR2_dsRNA_R CGGCTTGATGTGCTGAATTAC
TuRXR β _dsRNA_F TCCGTTTACCGATGCAAGAA 381
TuRXR β _dsRNA_R GTGGAACGACTCAAGGGTTAT
TuSRC_dsRNA_F CGTGACATGCCGAAGAAGATA 355
TuSRC_dsRNA_R TACCAAGGGCAGACATAGGA
TuCBP_dsRNA_F ACCCAGTCACCCAATGTATC 334
TuCBP_dsRNA_R AAGATGGTGGTGGAGTGTATC
TuFaMet_dsRNA_F GGATTGATGCTGAAGGAGGT 329
TuFaMet_dsRNA_R ATGAGATCCTTGATGGAAGGTG
rp49_qRT_F CTTCAAGCGGCATCAGAGC 105
rp49_qRT_R CGCATCTGACCCTTGAACTTC
TuMet_qRT_F GGTGCGCTCCGATGAAATCAATGT 89
TuMet_qRT_R AGCCTAAGCTAGCGAACGCAGAAT
TuMet_Trans_F AGCAAGCTT ATGGCCACTGAGGAAACAATGG
TuMet_Trans_R TCGACTCGAGTTATTGTTTGAGATCTAGTTCGGGT

T7 promoter sequence TAATACGACTCACTATAGGG was added at the 5′ end of each dsRNA primer.