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. 2018 Oct 2;8:14713. doi: 10.1038/s41598-018-32922-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In silico analyses of Cdf79930. (A) Phylogenetic analysis of ulvan lyases. This phylogenetic tree was constructed using UPGMA based on a Kimura 2p distance matrix using the program CLC Sequence Viewer ver. 8.0. Numbers indicate the bootstrap values. (B) Structures of ulvan lyases. (C) The CD spectrum of Cdf79930. The CD measurements were carried out using 0.1 mg ml⁻¹ solution of Cdf79930 in 10 mM Tris buffer (pH 8). The average of the three scans was plotted, and the secondary structure (inset) was calculated using the K2D2 server.