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. 2018 Oct 2;9:4043. doi: 10.1038/s41467-018-06451-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 9 — Effect of type II surface mutations on NOD2 dependent NF-κB signalling. a Domain organization of the HA-RIP2fl construct used for the luciferase reporter assay. b, d Luciferase reporter assay in HEK293T cells showing the effect on NF-κB signalling of transfected WT and mutant RIP2fl for (b) type IIb surface and (d) type IIa surface. Each MDP value is significantly higher than that using c MDP. Average RLU values for WT RIP2fl-MDP and control-MDP are shown by dashed red and blue lines, respectively. The boxplot is the graphical representation of the summary statistics of a vector contains information in this order: minimum value (lower whisker), first quartile (25% of data lower bound box), median (50% of data centre line), third quartile (75% of data higher bound box) and maximum value (upper whisker). Black dots on boxplot are extreme values. c, e Example WB for (c) type IIb surface mutants and (e) type IIa surface mutants, showing expression of wild-type (WT) HA-RIP2fl and mutants (top) and β-actin (bottom). For each construct, the results for cells induced by either MDP or its control (cMDP, c) are reported in black and cyan respectively