Fig. 1.

Spindle migration following anaphase-onset induces protrusion. a–c Stages of anaphase (a) and quantification of stage-specific spindle dimensions (b, c) derived from live oocytes with fluorescently labelled spindles and chromosomes. One-way ANOVA used for statistical analysis. d, e Schematics depicting stages of anaphase (d) and measurements (e). f Example of protrusion (white arrowheads) and spindle migration into the membrane occurring after anaphase-onset in a live oocyte (see Supplementary Movie 3). g–i Quantification of protrusion height, spindle displacement and oocyte dimensions (g, n = 12, error bars are mean ± SEM) (see e); total posterior pole displacement (h) and comparison with total midzone displacement (i) between anaphase-onset and anaphase-completion. Two-tailed Student’s t test used for statistical analysis. j Correlation between spindle migration and protrusion height (n = 12). Times in panels are hours:minutes relative to anaphase-onset. Scale bars, 10 µm. Box plots depict median (horizontal line), mean (crosses), 25th and 75th percentiles (boxes) and 5th and 95th percentiles (whiskers). Oocyte numbers are shown in parenthesis. P values represented as **P < 0.01, ***P < 0.001 and ****P < 0.0001, ns denoted P > 0.05