Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Oct 2;8:14662. doi: 10.1038/s41598-018-33044-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Disulfide-based dimerization of AnxA2 affects membrane bridging/linking. (A) Frequency and dissipation shifts monitored in the QCM-D setup depicted in Fig. 1 that are induced by WT AnxA2 following incubation of the protein at non-reducing conditions for different periods of time [week: 1 (black), 3 (blue) and 5 (red)]. (B) SDS-PAGE analysis of different AnxA2 derivates under non-reducing conditions: 1, alkylated WT AnxA2; 2, WT AnxA2 (oxidation state after 1 week); 3, AnxA2 Δ32; 4, WT AnxA2 (oxidation state after 5 weeks); 5, AnxA2 Δ14. PageRuler™ Plus Prestained Protein Ladder was used as a molecular weight marker. (C) Mass analyses of non-modified WT AnxA2 (black graph) and the alkylated derivative (blue graph). (D) Time-resolved frequency (i) and dissipation (ii) monitoring of the reversible, Ca²⁺ dependent binding of alkylated AnxA2. A model representing the respective experimental setup is shown below the QCM-D recordings (iii). Shown are representative examples of a typical experiment carried out n = 8 independent times with two different protein batches and vesicle preparations.