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. 2018 Oct 2;8:14711. doi: 10.1038/s41598-018-32822-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of expressed fusion and purified pBD2 and its antimicrobial activity. (A) Analysis of expressed fusion pBD2. Lane 1 indicates the induced protein; Lane 2 indicates the protein after boiling at 80 °C for 10 min; Lane 3 indicates the protein marker; Lanes 4–7 indicate the 4–7 fractions after purification of pBD2 by HisTrapTMHP chromatography; (B) Analysis of the purified protein. Lane 1 indicates the protein marker; Lanes 2–3 indicate the purified fusion protein after enrichment; (C) Antimicrobial activities of pBD2 against approximately 10⁹ cfu/mL E. coli at different time points. OD values at 630 nm (reference 405 nm) were measured at different times at various pBD2 concentrations. The OD value at 0 h was adjusted to 0, and the increased OD values were calculated. The full-length gels are presented in Supplementary Figure 1.