Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Oct 2;9:4027. doi: 10.1038/s41467-018-06334-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — EPCAM+/CD271+ cells are hair cell progenitors. a Schematic illustration of EPCAM and CD271 expression in the cochlear duct. Double-positive cells in the PSD are shown in yellow. FACS plot showing the gated populations from a W12 sample (E1242). b Pie chart illustrating the distribution of the four FACS-sorted populations. n = 8 fetal tissues (week 9.4 to 12.3). Values are mean percent ± s.d. c Quantification of the total number of hair cells-like cell (HC) per sample obtained from the two sorted populations (N = 5 samples: W9.7 (E1238), W10.5 (E1270), W12.5 (E1242), W11 (E1254), and W12 (E1289)). EPCAM+/CD271+ cells: N = 19 organoids; EPCAM+/CD271− cells: n = 47 organoids.. Box plot indicates distribution, middle line: median, whiskers: minimum and maximum. d Representative examples of organoids derived from EPCAM+/CD271+ and e EPCAM+/CD271− populations (W12 sample, E1242). Immunostaining for MYO7A and co-staining with phalloidin and DAPI is shown. Selected hair cells-like cell morphologies from the 3D stack are shown in d. Scale bars = 100 μm. f Organoid derived from EPCAM+/CD271+ cells from a W9.7 sample (E1238) stained for MYO7A, F-actin and DAPI. Scale bar 100 μm. The area marked by the asterisk is enlarged in the right panel. g Organoid derived from EPCAM+/CD271+ cells from a W10.5 sample (E1270) immunostained for MYO7A. Scale bar = 100 μm. The same organoid is shown at higher magnification in h. h The sample is re-stained for ESPIN (red) and F-actin. Scale bar 10 μm