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. 2018 Oct 2;8:14645. doi: 10.1038/s41598-018-32905-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cellular ultrastructure observation. (A,B) Phenotypes of wild-type strain Yb2. (A) Integral and regularly shaped cells, a few cells were in division; (B) regularly shaped cells at higher magnification. (C,D) Phenotypes of complemented strain cYb2ΔbioF. (C) Integral and regularly shaped cells; (D) regularly shaped cells at higher magnification. (E–H) Phenotypes of mutant strain Yb2ΔbioF. (E) Dropped vegetations (red arrows) and most cells were in division (black arrows); (F) relatively thickened cell walls (black arrow); (G) partially thickened cell walls (black arrow); (H) irregular shapes (black arrow).