Table 1.

Real-time PCR verification of differentially expressed genes in mutant strain Yb2ΔbioF.

Gene locusa	Description of genes	2−ΔΔCt b
AS87_RS05800	hypothetical protein	1.69
AS87_RS04150	glycoside hydrolase	1.55
AS87_RS04120	exosortase F system-associated protein	1.53
AS87_RS09460	hypothetical protein	1.51
AS87_RS09445	hypothetical protein	1.34
AS87_RS03745	NADH-quinone oxidoreductase subunit A	1.29
AS87_RS05725	hypothetical protein	1.27
AS87_RS05655	haloacid dehalogenase	1.16
AS87_RS02380	ABC transporter ATP-binding protein	1.14
AS87_RS06190	ABC transporter ATP-binding protein	1.13
AS87_RS03960	type B 50S ribosomal protein L31	0.99
AS87_RS01395	transcription activator effector-binding protein	0.97
AS87_RS10270	hypothetical protein	0.96
AS87_RS02825	HIT family protein	0.96
AS87_RS07030	mannose-6-phosphate isomerase	0.95
AS87_RS10115	hypothetical protein	0.91
AS87_RS01890	hypothetical protein	0.86
AS87_RS10350	IS982 family transposase	0.86
AS87_RS06890	hypothetical protein	0.85
AS87_RS02260	polyphosphate kinase 2	0.84
AS87_RS01190	DUF465 domain-containing protein	0.79
AS87_RS00965	50S ribosomal protein L19	0.75
AS87_RS05265	hypothetical protein	0.67
AS87_RS09430	hypothetical protein	0.61
AS87_RS07650	30S ribosomal protein S20	0.60
AS87_RS00160	monofunctional biosynthetic peptidoglycan transglycosylase	0.54
AS87_RS08680	TonB-dependent receptor	0.51
AS87_RS02480	hypothetical protein	0.50
AS87_RS09175	dethiobiotin synthase	0.50
AS87_RS01370	VIT family protein	0.48
AS87_RS07305	cytochrome c nitrite reductase small subunit	0.42
AS87_RS01365	sulfite exporter TauE/SafE family protein	0.42
AS87_RS01360	efflux RND transporter periplasmic adaptor subunit	0.35
AS87_RS01355	TolC family protein	0.35
AS87_RS01350	CusA/CzcA family heavy metal efflux RND transporter	0.34
AS87_RS10025	hypothetical protein	0.31
AS87_RS07820	alpha/beta hydrolase	0.27
AS87_RS09180	adenosylmethionine–8-amino-7-oxononanoate aminotransferase BioA	0.20
AS87_RS09185	hypothetical protein	0.15

^aBased on Riemerella anatipestifer Yb2 genome (accession number: CP007204).

^bResults are presented as 2^ΔΔCt. Figure = 1 indicated that the gene is expressed similarly in both mutant strain Yb2ΔbioF and wild-type strain Yb2, figures >1 indicated that the gene is over expressed in mutant strain Yb2ΔbioF, and figures <1 indicated that the gene is expressed less in mutant strain Yb2ΔbioF.