Table 2.
Strains, plasmids and primers used in this study.
| Strains, plasmids or primers | Characteristics | Sources or references |
|---|---|---|
| Strains | ||
| Yb2 | Riemerella anatipestifer serotype 2 strain | 39 |
| Yb2ΔbioF | Tn4351 insertion mutant of R. anatipestiferYb2, bioF::Tn | 17 |
| cYb2ΔbioF | Mutant strain RA1893 carrying plasmid pCP29-bioF | This study |
| Escherichia coli S17-1 | λpirhsdRprothi; chromosomally integrated RP4-2, Tc::Mu Km::Tn7 | 7 |
| Plasmids | ||
| pCP29 | ColE1 ori (pCP1 ori); Apr(Emr); E. coli-F. johnsoniae shuttle plasmid | 7 |
| pCP29-bioF | pCP29 containing ompA promoter and bioF ORF, cfxAr(Apr) | This study |
| Primers | ||
| ompA promoter P1 | 5′-CAGGTACCATAGCTAAAATTTTGGCAGTAAC-3′ (KpnΙ site underlined) | 7 |
| ompA promoter P2 | 5′-CGACTCGAGCATTCCAATTCTCTTATTATC-3′ (XhoΙ site underlined) | 7 |
| 16S rRNA F | 5′-GAGCGGTAGAGTATCTTCGGATACT-3′ | 8 |
| 16S rRNA R | 5′-AATTCCTTTGAGTTTCAACCTTGCG-3′ | 8 |
| bioF comp-F | 5′-CCGCTCGAGATGAATATTCCCAAAAGATTGTTAGAT-3′ (XhoΙ site underlined) | This study |
| bioF comp-R | 5′-CATGCATGCTTATTTCTTTAAATTAGAAATGATAAAATTGTGGGC-3′ (SphΙ site underlined) | This study |