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. 2018 Sep 26;9:1084. doi: 10.3389/fphar.2018.01084

FIGURE 4.

FIGURE 4

Glucuronidation of 4OH- and 5OH-AB-PINACA in human liver microsomes (HLMs). 4OH-AB-PINACA (A) and 5OH-AB-PINACA (B) were incubated for 90 min with HLMs in the presence or absence of UDPGA. The terminated reaction mixtures were subjected to the UPLC analysis and the eluent was monitor with a UV detector at 300 nm and chromatograms presented. (C) The peak area of the glucuronide was estimated from the triplicate determinations. The data are presented as mean value ± SD. ND, not detected. (D) Comparison of glucuronidation of JWH-018-M2 and 4OH-AB-PINACA with HLMs. JWH-018-M2 and 4OH-AB-PINACA (5 μM) were incubated for 90 min with HLMs in the presence or absence of UDPGA. The terminated reaction mixtures were subjected to the UPLC analysis and the eluent was monitor with a UV detector at 300 nm. The remaining amount of the substrates was quantified by using standard curves of JWH-018-M2 and 4OH-AB-PINACA. The data was shown as mean value ± SD. ∗P < 0.001.