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. 2018 Sep 26;9:2216. doi: 10.3389/fimmu.2018.02216

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Copyright © 2018 Wang, Wu, Pan, Chen, Goto and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Images of the 2-DE oxyblot. Analysis of protein oxidation levels in DNP-derivatized proteins among syngenic control, POD14 and POD63 samples. Obvious reduction in the carbonylation levels of proteins are observed in the tolerance group compared to rejection group. The quantitative results indicating the differentially carbonylated level of protein on syngenic control, POD14 and POD63 were demonstrated as the bar chart. (B) HepG₂ cell viability was determined under the treatments of 0, 1, 5 and 10 mM H₂O₂. The result was quantified and indicated by the bar chart. (C) Western blotting analysis for PCNA, caspase-3, ceruloplasmin and SOD-1 levels under treatment of different concentrations of H₂O₂. The relative expression ratio to β-actin is shown at the bottom.