Table 2.
Live imaging results after treatment of heat activated Bacillus subtilis strains 168 dormant spores with the peptides.
Treatment | Start of germination (min) | Germination time (min) | Burst (min) | Generation time (min) | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Median | p-value | IQR | Counts | Median | p-value | IQR | Counts | Median | p-value | IQR | Counts | Median | p-value | IQR | Counts | ||
Untreated | 17 | 10 | 384 | 4 | 1 | 384 | 120 | 25 | 369 | 44 | 9 | 345 | |||||
TC19 | Sub-lethal | 16 | 0.34 | 13 | 174 | 4 | 0.03 | 2 | 174 | 142 | ≤0.01 | 38 | 52 | 44 | 0.59 | 5 | 49 |
Lethal | 18 | 0.08 | 10 | 370 | 4 | ≤0.01 | 2 | 370 | |||||||||
Coated | 14 | 0.03 | 12 | 228 | 4 | 0.01 | 1 | 228 | 110 | ≤0.01 | 25 | 168 | 42 | ≤0.01 | 3 | 151 | |
TC84 | Sub-lethal | 17 | 0.07 | 10 | 212 | 4 | ≤0.01 | 1 | 212 | 105 | ≤0.01 | 25 | 117 | 41 | ≤0.01 | 4 | 129 |
Lethal | 16 | 0.98 | 12 | 342 | 4 | ≤0.01 | 2 | 342 | |||||||||
Coated | 18 | ≤0.01 | 15 | 209 | 4 | ≤0.01 | 1 | 209 | 107 | ≤0.01 | 39 | 188 | 40 | ≤0.01 | 3 | 177 | |
BP2 | Sub-lethal | 15 | 0.17 | 11 | 380 | 4 | ≤0.01 | 1 | 380 | 115 | 0.03 | 27 | 317 | 53 | ≤0.01 | 33 | 288 |
Lethal | 18 | 0.02 | 13 | 499 | 4 | ≤0.01 | 2 | 499 | |||||||||
Coated | 16 | 0.89 | 13 | 325 | 4 | ≤0.01 | 1 | 325 | 110 | ≤0.01 | 28 | 270 | 43 | 0.47 | 7 | 210 | |
Nisin A | Sub-lethal | 16 | 0.7 | 13 | 169 | 4 | ≤0.01 | 1 | 169 | 114 | 0.19 | 28 | 52 | 39 | ≤0.01 | 3 | 52 |
Lethal | 16 | 0.26 | 12 | 354 | 3 | ≤0.01 | 1 | 354 | |||||||||
Coated | 15 | 0.68 | 12 | 169 | 4 | ≤0.01 | 1 | 169 | 113 | 0.43 | 48 | 33 | 40 | ≤0.01 | 3 | 28 | |
Coated and lethal concentrations were 32 μM TC19, 23 μM TC84, 56 μM BP2, and 7 μM Nisin A.
Sub-lethal concentrations were 16 μM TC19, 12 μM TC84, 28 μM BP2, and 3.5 μM Nisin A.
IQR refers to inter-quartile range.