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. 2018 Sep 26;9:2277. doi: 10.3389/fmicb.2018.02277

Table 2.

Live imaging results after treatment of heat activated Bacillus subtilis strains 168 dormant spores with the peptides.

Treatment Start of germination (min) Germination time (min) Burst (min) Generation time (min)




Median p-value IQR Counts Median p-value IQR Counts Median p-value IQR Counts Median p-value IQR Counts
Untreated 17 10 384 4 1 384 120 25 369 44 9 345

TC19 Sub-lethal 16 0.34 13 174 4 0.03 2 174 142 ≤0.01 38 52 44 0.59 5 49
Lethal 18 0.08 10 370 4 ≤0.01 2 370
Coated 14 0.03 12 228 4 0.01 1 228 110 ≤0.01 25 168 42 ≤0.01 3 151
TC84 Sub-lethal 17 0.07 10 212 4 ≤0.01 1 212 105 ≤0.01 25 117 41 ≤0.01 4 129
Lethal 16 0.98 12 342 4 ≤0.01 2 342
Coated 18 ≤0.01 15 209 4 ≤0.01 1 209 107 ≤0.01 39 188 40 ≤0.01 3 177
BP2 Sub-lethal 15 0.17 11 380 4 ≤0.01 1 380 115 0.03 27 317 53 ≤0.01 33 288
Lethal 18 0.02 13 499 4 ≤0.01 2 499
Coated 16 0.89 13 325 4 ≤0.01 1 325 110 ≤0.01 28 270 43 0.47 7 210
Nisin A Sub-lethal 16 0.7 13 169 4 ≤0.01 1 169 114 0.19 28 52 39 ≤0.01 3 52
Lethal 16 0.26 12 354 3 ≤0.01 1 354
Coated 15 0.68 12 169 4 ≤0.01 1 169 113 0.43 48 33 40 ≤0.01 3 28

Coated and lethal concentrations were 32 μM TC19, 23 μM TC84, 56 μM BP2, and 7 μM Nisin A.

Sub-lethal concentrations were 16 μM TC19, 12 μM TC84, 28 μM BP2, and 3.5 μM Nisin A.

IQR refers to inter-quartile range.