Figure 6.

Binding of the C‐terminal segment and ColG CBDs with or without calcium ions. Five micrograms of the C‐terminal segment or 5 μg each of ColG CBDs (s3b and s3a3b) in 50 μL of the binding buffer was incubated with (+) and without (−) 2.5 mg of insoluble type I collagen fibers when varying Ca²⁺ ion concentration (5 mm Ca²⁺ ion or 5 mm Ca²⁺ ion with 10 mm EGTA). After incubation, the filtrates were analyzed by SDS/PAGE using a 4–20% gradient polyacrylamide gel. Numbers on the left are molecular masses (in kDa) of the markers.