Skip to main content
. 2018 Sep 8;8(10):1636–1648. doi: 10.1002/2211-5463.12504

Figure 5.

Figure 5

Confirmation of RBL interactors and subcellular localization of interactors. (A) Direct yeast two‐hybrid experiments confirming interactions of RBL with VFP3/ENAP1 and OBE1. Negative controls included tests with empty vectors (pAS2‐1) and with a cDNA in which a nucleotide had been deleted, inducing a frameshift mutation (VFP3/ENAP1del, OBE1del). Yeasts were grown on SD‐AHTL selective media. (B) Localizations of the chimeric proteins OBE1‐GFP and VFP3/ENAP1‐GFP in Arabidopsis thaliana root cells. DNA is stained with DAPI. (C) Localization of VFP3/ENAP1‐GFP in tobacco leaf cells. The splicing speckles are stained with cyc64‐mRFP marker. Scale bar: 10 μm.