Table 1. Macromolecule-production information.

Source organism	S. cerevisiae (strain W303)
DNA source	Genomic DNA
Forward primer†	5′-CTCCTGGATCCATGGTCAAAGTCGCAATT-3′
Reverse primer‡	5′-CCAGCGTCGACTCATAGCTTGGAAGAGTC-3′
Cloning vector	pCold I
Expression vector	pCold I
Expression host	E. coli BL21 (DE3)
Complete amino-acid sequence of the construct produced§	MNHKVHHHHHHIEGRHMDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQAPEDLDMEDNDIIEAHREQIGGGSMVKVAILGASGGVGQPLSLLLKLSPYVSELALYDIRAAEGIGKDLSHINTNSSCVGYDKDSIENTLSNAQVVLIPAGVPRKPGLTRDDLFKMNAGIVKSLVTAVGKFAPNARILVISNPVNSLVPIAVETLKKMGKFKPGNVMGVTNLDLVRAETFLVDYLMLKNPKIGQEQDKTTMHRKVTVIGGHSGETIIPIITDKSLVFQLDKQYEHFIHRVQFGGDEIVKAKQGAGSATLSMAFAGAKFAEEVLRSFHNEKPETESLSAFVYLPGLKNGKKAQQLVGDNSIEYFSLPIVLRNGSVVSIDTSVLEKLSPREEQLVNTAVKELRKNIEKGKSFILDSSKL

^†The BamHI site is underlined.

^‡The SalI site is underlined.

^§The hexahistidine–SUMO tag is italicized.