Table 2. Crystallization.
| Apo form | NAD+ complex | NAD+/OAA complex | |
|---|---|---|---|
| Method | Sitting-drop vapor diffusion | ||
| Plate type | 96-well 2-drop MRC crystallization plates | ||
| Temperature (K) | 293 | ||
| Protein concentration (mg ml−1) | 16.2 | ||
| Buffer composition of protein solution | 25 mM Tris–HCl pH 7.5, 150 mM NaCl, 1 mM DTT | ||
| Composition of reservoir solution | 0.1 M CHES pH 9.5, 30%(v/v) PEG 400 | 0.1 M bis-Tris pH 5.5, 0.2 M MgCl2, 25%(w/v) PEG 3350 | 0.1 M HEPES pH 7.5,20%(w/v) PEG 10 000 |
| Volume and ratio of drop | 1 µl:1 µl | ||
| Volume of reservoir (µl) | 50 | ||