Table 3. Data collection and processing.
Values in parentheses are for the outer shell.
| Apo form | NAD+ complex | NAD+/OAA complex | |
|---|---|---|---|
| Diffraction source | BL44XU, SPring-8 | BL44XU, SPring-8 | BL44XU, SPring-8 |
| Wavelength (Å) | 0.900 | 0.900 | 0.900 |
| Temperature (K) | 100 | 100 | 100 |
| Detector | MX300-HE CCD | MX300-HE CCD | MX300-HE CCD |
| Crystal-to-detector distance (mm) | 300 | 280 | 250 |
| Rotation range per image (°) | 0.5 | 0.5 | 0.5 |
| Total rotation range (°) | 180 | 180 | 180 |
| Exposure time per image (s) | 0.5 | 0.5 | 0.5 |
| Space group | P212121 | P21 | P212121 |
| Unit-cell parameters (Å, °) | a = 57.5, b = 86.6, c = 132.9 | a = 45.1, b = 92.6, c = 82.1, β = 99.9 | a = 57.1, b = 86.7, c = 135.1 |
| Mosaicity (°) | 0.65–0.83 | 1.46–1.85 | 0.6–1.06 |
| Resolution range (Å) | 50–2.10 (2.14–2.10) | 50–2.00 (2.03–2.00) | 50–2.10 (2.14–2.10) |
| Total No. of reflections | 261381 | 157113 | 279474 |
| No. of unique reflections | 39231 | 44896 | 40043 |
| Completeness (%) | 99.0 (100.0) | 99.8 (100.0) | 99.9 (100.0) |
| Multiplicity | 6.7 (6.3) | 3.5 (3.4) | 7.0 (6.8) |
| 〈I/σ(I)〉 | 60.1 (10.5) | 30.4 (4.0) | 40.0 (5.7) |
| R p.i.m. | 0.034 (0.143) | 0.048 (0.329) | 0.028 (0.186) |
| Overall B factor from Wilson plot (Å2) | 29.3 | 22.0 | 27.0 |