| Method |
Sitting-drop vapour diffusion for initial screening, hanging-drop vapour diffusion for crystal optimization |
Sitting-drop vapour diffusion |
| Plate type |
96-well plate for initial crystal screening, 24-well plates for crystal optimization |
96-well plate |
| Temperature (K) |
293 |
293 |
| Protein concentration (mg ml–1) |
6 |
6 |
| Buffer composition of protein solution |
10 mM MES pH 6.8 |
10 mM MES pH 6.8 |
| Composition of reservoir solution |
0.1 M Tris–HCl pH 7.0, 0.2 M MgCl2, 9%(w/v) PEG 8000 |
0.1 M HEPES pH 7.5, 0.1 M CaCl2, 8.25%(w/v) PEG 3350, 1.32%(v/v) 2-propanol |
| Volume and ratio of drop |
0.5 µl, 1:1 for initial screening; 1 µl, 1:1 for crystal optimization |
0.5 µl, 1:1 |
| Volume of reservoir (µl) |
50 µl for initial screening, 1000 µl for crystal optimization |
50 µl for initial screening |
